Journal: Journal of Microbiology and Biotechnology

Article Title: Korean Red Pine ( Pinus densiflora ) Bark Extract Attenuates Aβ-Induced Cognitive Impairment by Regulating Cholinergic Dysfunction and Neuroinflammation

doi: 10.4014/jmb.2207.07015

Figure Lengend Snippet: ( A ) Western blot band image, Protein expression levels of ( B ) p-CREB, and ( C ) BDNF. The results shown are mean ± SD ( n = 3). Data were statistically considered at p < 0.05, and different small letters represent statistical difference.

Article Snippet: Brain-derived neurotrophic factor (BDNF) (CSB-PA05775A0Rb) was purchased from Cusabio Biotech (China), and anti-phospho-cAMP response element-binding protein (p-CREB) (9198) and anti-rabbit (7074S) were purchased from Cell Signaling Technology (USA).

Techniques: Western Blot, Expressing

Journal: European Archives of Psychiatry and Clinical Neuroscience

Article Title: Brain-derived neurotrophic factor-TrkB signaling in the medial prefrontal cortex plays a role in the anhedonia-like phenotype after spared nerve injury

doi: 10.1007/s00406-018-0909-z

Figure Lengend Snippet: Levels of proBDNF in selected tissues among the sham, anhedonia susceptible, and unsusceptible groups. a mPFC ( F (2,15) = 15.179, P = 0.001). b NAc ( F (2,15) = 24.61, P < 0.001). c Hippocampus ( F (2,15) = 59.837, P < 0.001). d L2–5 spinal cord ( F (2,15) = 3.021, P = 0.079). e Muscle ( F (2,15) = 2.466, P = 0.119). f Liver ( F (2,15) = 6.11, P = 0.031). Data are shown as mean ± S.E.M. ( n = 6). * P < 0.05, ** P < 0.01 or *** P < 0.01. proBDNF precursor of BDNF, mPFC medial prefrontal cortex, NAc nucleus accumbens, N.S . not significant

Article Snippet: Relative primary antibodies were incubated at 4 °C overnight: rabbit mature BDNF (1:500, Sangon Biotech, Shanghai, China), rabbit proBDNF (1:500; Origene, Rockville, MD, USA), rabbit TrkB (1:1000, Cell Signaling Technology, Danvers, MA, USA), rabbit phosphorylated p-TrkB (1:1000, Affinity, Cincinnati, OH, USA), and mouse GAPDH (1:1000, Qidongzi, Wuhan, China).

Techniques:

Journal: European Archives of Psychiatry and Clinical Neuroscience

Article Title: Brain-derived neurotrophic factor-TrkB signaling in the medial prefrontal cortex plays a role in the anhedonia-like phenotype after spared nerve injury

doi: 10.1007/s00406-018-0909-z

Figure Lengend Snippet: Effects of 7,8-DHF and ANA-12 on BDNF-TrkB signaling in mPFC and NAc in anhedonia susceptible rats. a Western blot bands of BDNF-TrkB signaling in the mPFC. b BDNF level in the mPFC ( F (3,20) = 119.273, P < 0.001). c proBDNF level in the mPFC ( F (3,20) = 99.914, P < 0.001). d p-TrkB/TrkB ratio in the mPFC ( F (3,20) = 22.731, P < 0.001). e TrkB level in the mPFC ( F (3,20) = 0.340, P = 0.797). f Western blot bands of BDNF-TrkB signaling in the NAc. g BDNF level in the NAc ( F (3,20) = 17.414, P = 0.001). h proBDNF level in the NAc ( F (3,20) = 21.974, P < 0.001). i p-TrkB/TrkB ratio in the NAc ( F (3,20) = 10.828, P = 0.003). j TrkB level in the NAc ( F (3,20) = 0.140, P = 0.933). Data are shown as mean ± S.E.M. ( n = 6). BDNF brain-derived neurotrophic factor, mPFC medial prefrontal cortex, NAc nucleus accumbens, N.S . not significant, p-TrkB phosphorylation-tropomyosin-receptor kinase B, proBDNF precursor of BDNF; * P < 0.05, ** P < 0.01 or *** P < 0.001

Article Snippet: Relative primary antibodies were incubated at 4 °C overnight: rabbit mature BDNF (1:500, Sangon Biotech, Shanghai, China), rabbit proBDNF (1:500; Origene, Rockville, MD, USA), rabbit TrkB (1:1000, Cell Signaling Technology, Danvers, MA, USA), rabbit phosphorylated p-TrkB (1:1000, Affinity, Cincinnati, OH, USA), and mouse GAPDH (1:1000, Qidongzi, Wuhan, China).

Techniques: Western Blot, Derivative Assay, Phospho-proteomics

Journal: Journal of Biological Chemistry

Article Title: N-Glycosylation is required for secretion of the precursor to brain-derived neurotrophic factor (proBDNF) carrying sulfated LacdiNAc structures

doi: 10.1074/jbc.ra119.009989

Figure Lengend Snippet: Figure 6. Extracted ion chromatograms of four most abundant glycopeptides of proBDNF expressed in PC12 cells. (A, C, D) LacdiNAc containing structures, (B) LacNAc containing structure. Arrow points to the peaks of glycopeptides aligned with slight retention time shifts as expected based on the hydrophilicity of the attached glycan. Numbers represent intensity of the extracted peaks (x 105).

Article Snippet: Expression vector pCMV6-Entry bearing human proBDNF cDNA (NM_001709, Origene, Rockville, MD) with C-terminal Myc-DDK tags was modified to introduce 6xHis affinity tag between signal peptide sequence and proBDNF polypeptide chain using QuikChange Lightning Multi Site-Directed Mutagenesis kit (Agilent Technologies, Santa Clara, CA) according to manufacturer’s instructions and mutagenic primers 5’-CAT ACT TTG GTT GCA TGA AGG CTC ACC ATC ACC ACC ATC ACG CCC CCA TGA AA-3’ (sense) and 5’-TTT CAT GGG GGC GTG ATG GTG GTG ATG GTG AGC CTT CAT GCA ACC AAA GTA TG-3’.

Techniques: Glycoproteomics

Journal: International Journal of Neuropsychopharmacology

Article Title: Sustained Ultrastructural Changes in Rat Hippocampal Formation After Repeated Electroconvulsive Seizures

doi: 10.1093/ijnp/pyaa021

Figure Lengend Snippet: Brain-derived neurotrophic factor (BDNF) expression levels were measured by immunohistochemistry in hippocampus. (A) BDNF expression in the subregions of hippocampus. (B–D) Immunohistochemistry examined BDNF expression levels in each group. Mean optical density (MOD) was calculated with the following formula: OD = log10(max pixel intensity/mean pixel intensity), where max pixel intensity = 255. A single ECS treatment significantly increased the MOD of BDNF expression in both FSL and FRL rat strains in CA1 of hippocampus in contrast to DG and CA2/3 of hippocampus. No difference between the FRL sham and FSL sham rats was observed. Three months after 10xECS, no changes of BDNF expression levels in hippocampal subregions between the FRL and FSL rats were observed.

Article Snippet: Tissue sections were incubated at 4°C overnight in a solution containing rabbit anti-BDNF polyclonal antibody (1:500) (AB1779, Merck Millipore) or mouse anti-VEGF monoclonal antibody (1:400) (sc-53462, Santa Cruz Biotechnology, Inc.).

Techniques: Derivative Assay, Expressing, Immunohistochemistry

Journal: International Journal of Neuropsychopharmacology

Article Title: Sustained Ultrastructural Changes in Rat Hippocampal Formation After Repeated Electroconvulsive Seizures

doi: 10.1093/ijnp/pyaa021

Figure Lengend Snippet: Results of All Groups in FSL Rats

Article Snippet: Tissue sections were incubated at 4°C overnight in a solution containing rabbit anti-BDNF polyclonal antibody (1:500) (AB1779, Merck Millipore) or mouse anti-VEGF monoclonal antibody (1:400) (sc-53462, Santa Cruz Biotechnology, Inc.).

Techniques:

Journal: International Journal of Neuropsychopharmacology

Article Title: Sustained Ultrastructural Changes in Rat Hippocampal Formation After Repeated Electroconvulsive Seizures

doi: 10.1093/ijnp/pyaa021

Figure Lengend Snippet: Results of All Groups in FRL Rats

Article Snippet: Tissue sections were incubated at 4°C overnight in a solution containing rabbit anti-BDNF polyclonal antibody (1:500) (AB1779, Merck Millipore) or mouse anti-VEGF monoclonal antibody (1:400) (sc-53462, Santa Cruz Biotechnology, Inc.).

Techniques:

Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

Article Title: Testosterone depletion in adult male rats increases mossy fiber transmission, LTP, and sprouting in area CA3 of hippocampus

doi: 10.1523/JNEUROSCI.3857-12.2013

Figure Lengend Snippet: A. BDNF-ir in a sham (1) and Gdx rat (2) using a rabbit polyclonal antibody to BDNF (provided by Amgen-Regeneron Partners). DG=dentate gyrus. Arrows point to the MF pathway. Calibration = 500 μm.

Article Snippet: Initial experiments used a well-described polyclonal antibody to BDNF ( Conner et al., 1997 ; Yan et al., 1997 ) and showed that Gdx rats (which had surgery at approximately 60 days of age and were evaluated approximately 2 weeks later) exhibited greater BDNF-ir in the MFs than sham rats ( ). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 8 caption a7 caption a8 Gdx rats exhibit increased BDNF-ir in the MF pathway A. BDNF-ir in a sham (1) and Gdx rat (2) using a rabbit polyclonal antibody to BDNF (provided by Amgen-Regeneron Partners).

Techniques: